Cost effective production of Bacillus thuringiensis subsp. israelensis using chemically pre-treated rice straw

Bacillus thuringiensis subsp. israelensis is the leading biopesticide in world due to its specificity and lack of toxicity. The use Bti still restricted developing countries bioprocess complications high cost production. In present study we have shown that lignocellulosic agro waste residues like rice straw can be used as effective substrates with appropriate chemical treatment methods. We compared untreated straw, pre-treated gives better biomass production, also shows less sporulation time, which turn reduced larval killing time part larvicidal assay. Sodium hydroxide (NaOH) has proven a delignifying agent Calcium (CaOH). It found supplementation medium fish slurry enhances production case both NaOH CaOH. This enhancement visible when chicken feather hydrolysate Nitrogen supplement, although it not hydrolysate.